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In
recent years there has been a huge advance in techniques that can
visualize key events in the nuclei of single cells. These include
primary transcription from individual alleles, and associated processes
such as chromatin remodelling and relocalization of DNA loci, and
localization of protein factors involved in gene activation and
silencing. The techniques include RNA-DNA -and - immuno-FISH
(fluorescence in situ hybridization) respectively. This meeting will
explore the practical application of these techniques, as well as the
tremendous advances that they have enabled, particularly in the
immunology field. They have identified new mechanisms such as
intergenic transcription, locus contraction and allele-specific
heterochromatinisation in V(D)J recombination and allelic exclusion.
They are particularly powerful for analysis of nuclear reorganisation
and gene expression changes during immune cell development, and for
study of monoallelic expression, for example for NK receptors,
interleukins, antigen receptors.
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