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In recent years there has been a huge advance in techniques that can visualize key events in the nuclei of single cells. These include primary transcription from individual alleles, and associated processes such as chromatin remodelling and relocalization of DNA loci, and localization of protein factors involved in gene activation and silencing. The techniques include RNA-DNA -and - immuno-FISH (fluorescence in situ hybridization) respectively. This meeting will explore the practical application of these techniques, as well as the tremendous advances that they have enabled, particularly in the immunology field. They have identified new mechanisms such as intergenic transcription, locus contraction and allele-specific heterochromatinisation in V(D)J recombination and allelic exclusion. They are particularly powerful for analysis of nuclear reorganisation and gene expression changes during immune cell development, and for study of monoallelic expression, for example for NK receptors, interleukins, antigen receptors.
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